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. 2005 Oct 10;391(Pt 2):343–350. doi: 10.1042/BJ20050404

Figure 2. Identification strategy for the peak IV uMUP.

Figure 2

The sequence data of uMUP peak IV peptides obtained by tandem MS (‘Q-ToF’) were used to interrogate the NCBI protein database using protein BLAST tool. A highly significant match was obtained for the GenBank® sequence XP_355497. From this sequence, the theoretical tryptic or endopeptidase LysC digestion maps were constructed, and used to identify those peptides (both partial and complete digestion fragments) observed during MALDI–TOF peptide mass fingerprinting. Tryptic peptides sequenced by tandem MS are aligned to the sequence – the symbol ‘J’ is used to express ambiguity between isobaric pair leucine and isoleucine.