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. 2005 Oct 10;391(Pt 2):399–408. doi: 10.1042/BJ20050795

Figure 3. Transient transfection analysis of the rat GCLM promoter–luciferase constructs in H4IIE cells.

Figure 3

Progressive 5′-deletions of the GCLM promoter extending from −1803 to +3 bp were generated and fused to the promoterless luciferase pGL-3 basic vector as described in the Materials and methods section. Numbering is defined relative to the translational start site. Results represent means±S.E.M. from four independent experiments performed in triplicates. TNFα treatment (15 ng/ml) was only during the last 4 h of the transfection. Data are expressed as relative luciferase activity compared with that of pGL-3 basic vector control, which is assigned a value of 1.0. *P<0.05 versus respective control GCLM (CON-GCLM) constructs (ANOVA followed by Fisher's test).