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. 2005 Oct 10;391(Pt 2):399–408. doi: 10.1042/BJ20050795

Figure 9. NF-kB positively regulates rat GCLM and GSS promoter activities, c-Jun expression and c-Jun-dependent promoter activity, and mediates c-Jun induction by TNFa.

Figure 9

Effect of p50 and p65 overexpression on GCLM and GSS promoter activity (A), c-Jun mRNA levels and c-Jun-dependent promoter activity (B). (C) The effect of blocking NF-κB with or without TNFα treatment on c-Jun mRNA level is shown. H4IIE cells were co-transfected with GCLM promoter construct −902/+3-LUC, GSS promoter construct −1164/+2-LUC or Jun2-Luc construct and p50 or p65 expression vector or empty vector control as described in the Materials and methods section. Overexpression of either p50 or p65 more than doubled the GCLM, GSS and c-Jun-dependent promoter activity (A, B), as well as increasing the steady state c-Jun mRNA levels (B). Baseline c-Jun level was 36% lower when NF-κB was blocked by IκBSR (C). TNFα treatment also increased the steady-state c-Jun mRNA level by 108%, which was significantly blocked by IκBSR (C). *P<0.05 versus respective controls (GCLM, GSS or Jun2-Luc). Con, control.