FIG. 3.
Effect of S. enterica serovar Typhimurium (S.ty) porins on ERK1/2 (A), p38 (B), and JNK (C) activation after different stimulation times. Proteins from cell lysates (3 × 106 cells/ml) were analyzed by SDS-15% PAGE and immunoblotted with an ERK1/2-, p38-, or JNK-phosphospecific antibody. Shifts in band mobility on SDS-PAGE due to phosphorylation were obtained with anti-ERK1/2, anti-p38, and anti-JNK. ERK1 appears as the upper band (44 kDa); ERK2 appears as the lower band (42 kDa). Fold increases in ERK1/2, p38, and JNK activation are shown below each lane for each blot. BSA represents a control of an unspecific stimulus.