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EMSA with AP-1 consensus oligonucleotide. U937 cells (3 × 106 cells/ml) were stimulated for 60 min with different porin concentrations. Control, 5 μg of untreated nuclear extract; Porins, 5 μg of nuclear extract induced with porins at 5 μg/ml, 1 μg/ml, and 100 ng/ml. The bracket at the left indicates control and induced complexes. Fold increases in AP-1 activation are shown below each lane for each blot.
