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Supershift with AP-1 consensus oligonucleotide. U937 cells (3 × 106 cells/ml) were stimulated for 60 min with porins (5 μg/ml) or LPS (1 μg/ml). Control, control nuclear extract (5 μg); LPS, LPS-induced nuclear extract (5 μg); Porins, porin-induced nuclear extract (5 μg). The specific antibodies used are indicated above the lanes. The arrows at the left indicate the specific supershifted complex, and the brackets indicate control and induced complexes.
