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. 2005 Oct 25;391(Pt 3):465–472. doi: 10.1042/BJ20050460

Figure 2. IR levels and activation in WT and NPC hepatocytes.

Figure 2

(A) WT and NPC hepatocytes were stimulated with insulin for 0–20 min and cell lysates were analysed by Western blotting for IR levels (with anti-IRβ antibody) and phosphorylation (with anti-pTyr antibody). Representative immunoblots (IB) after 5 min of insulin stimulation are shown. (B) Quantification of IR levels and (C) IR activation as a function of time. Values are means±S.E.M., n=44 in (B) and n=4–6 in (C). *P<0.05, **P<0.001, P values from a two-tailed Student's t test in all experiments.