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. 2005 Oct 25;391(Pt 3):481–490. doi: 10.1042/BJ20050897

Figure 3. Gel-filtration chromatography and native PAGE of NBD1+NBD2 mixture.

Figure 3

(A) Chromatographic profiles of Superdex 200 column fractions showing the elution of NBD1, 27 kDa; NBD2, 30 kDa; an NBD1+NBD2 mixture; and BSA, 67 kDa. The Superdex 200 column was calibrated with other proteins (indicated by arrows): 1, 43 kDa ovalbumin; 2, 25 kDa chymotrypsinogen A; 3, 13.7 kDa RNase A. Protein elution was recorded at A280. (B) Native PAGE was loaded with NBD2 (3 μg, lane 1), a mixture of NBD1 and NBD2 (3 μg for each NBD, lane 2) and NBD1 (3 μg, lane 3).