Figure 4. P- and L-selectin-binding activities of cells expressing the wt FucT-VII or its glycomutants.

CHO/C2P1 cells were transiently transfected by DNAs coding for the wt FucT-VII (F7 wt) or its glycomutants (N81Q, N291 and N81Q/N291Q) and incubated with the human IgM-conjugated P-selectin (left panel) or L-selectin (right panel). The binding was then revealed by fluorescence microscopy as in Figure 3(A). Gain and exposure time were kept constant between images.