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. 2005 Oct 25;391(Pt 3):491–502. doi: 10.1042/BJ20050344

Figure 5. Altered sLex and E-selectin binding to cells expressing the N-glycosylation-deficient variants of FucT-VII.

Figure 5

Cells expressing either the F7 wt or the mutated FucT-VII (N81Q, N291Q and N81Q/N291Q) were assayed for anti-sLex immunostaining using CSLEX-1 mAb (left panel) or E-selectin binding (right panel). The binding of CSLEX-1 mAb was revealed by an RITC (rhodamine isothiocyanate)-conjugated anti-mouse IgM and the binding of E-selectin–IgM chimaeras was revealed by an RITC-conjugated anti-human IgM. Immunostaining was then analysed by fluorescence microscopy. Gain and exposure time were kept constant between images.