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. 2005 Oct 25;391(Pt 3):491–502. doi: 10.1042/BJ20050344

Figure 6. Core2-modified PSGL-1 restores sLex expression and E-selectin binding activities of the mutated FucT-VII.

Figure 6

(A) CHO/C2P1 cells were transiently transfected with the wt FucT-VII or its double mutant N81Q/N291Q and analysed for E-selectin binding as in Figure 5. (B) Cells expressing C2GnT-I alone (CHO/C2), PSGL-1 alone (CHO/P1), or both molecules (CHO/C2P1), as well as those expressing PSGL-1 with the double mutant of C2GnT-I (CHO/dbmC2P1) were transiently transfected with the wt FucT-VII or its double mutant N81Q/N291Q and analysed for sLex expression as in Figure 5. The parental CHO cells (CHO) expressing none of these molecules were chosen as control. Gain and exposure time were kept constant between images. Cf, phase contrast.