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. 2005 Oct 25;391(Pt 3):491–502. doi: 10.1042/BJ20050344

Figure 7. Flow cytometric analyses of P-selectin binding to cells transfected with the wt or the doubly mutated FucT-VII.

Figure 7

CHO/C2P1 cells were transfected with constructs coding for the wt FucT-VII or its N81Q/N291Q double mutant, pretreated with increasing concentrations of the anti-PSGL-1 mAb PL1 and assayed for P-selectin binding as described in the Experimental section. (A) Dose-dependent inhibition by PL1 of P-selectin binding to cells expressing the wt FucT-VII (dotted grey lines) and to those expressing the N81Q/N291Q mutant (bold solid lines). (B) The dose–response curves deduced from the fluorescence data in (A) expressed as the percentage of the binding obtained in the absence of PL1. ■, The dose–response inhibition of P-selectin binding to cells expressing FucT-VII; ●, values from the double mutant.