Figure 8. PL1-mediated dose-dependent inhibition of P- and L-selectin binding.

Cells expressing the wild-type FucT-VII (wt) or the double mutant N81Q/N291Q were incubated with the human IgM-conjugated P-selectin (left panel) or L-selectin (right panel) in the absence (0 μg/ml) or in the presence of increasing concentrations of PL1 (from 0.2 to 8 μg/ml). The binding of the selectins was then examined by fluorescence microscopy as in Figure 4. Gain and exposure time were kept constant between images.