Table 1. Mutants and primers.

The sense and antisense primers for T60A correspond respectively to nt 151–192 and 127–168 of the C2GnT-I coding sequence and those for T97A correspond to nt 261–303 and 238–279 respectively. The sense and antisense primers for N81Q correspond respectively to nt 220–255 and 235–267 of the FucT-VII coding sequence and those for N291Q correspond to nt 850–891 and 860–903 respectively. In the sense primers of T60A and T97A, the codon ACC (coding for Thr) was replaced by GCC (coding for Ala). In the N81Q mutant of FucT-VII, the codon AAC (Asn) was replaced by CAG (Gln), and in N291Q the codon AAT (Asn) was replaced by CAA (Gln). The mutated nucleotides are italicized and marked by ‘*’. The overlapped sequences are underlined.

Species	Mutations	Primers
C2GnT-I	T60A	Sense: 5′-GAGAATCCTAGTAGTGATATTAATTGC*GCCAAAGTTTTACAG-3′
		Antisense: 5′-ATCACTACTAGGATTCTCCCCAGCAAGCTCCAAGTGTCTGAC-3′
	T97A	Sense: 5′-TGGACACCTGACGACTATATAAACATG*GCCAGTGACTGTTCT-3′
		Antisense: 5′-ATAGTCGTCAGGTGTCCACCGAGGGCGCTTTTTAAATTTCAC-3′
FucT-VII	N81Q	Sense: 5′-GCCCGCTGCCACCTGAGTGCC*CA*GCGAAGCCTG-3′
		Antisense: 5′-GTCGGCGCTGGCCAGCAGGCTTCG*CT*GGGCACT-3′
	N291Q	Sense: 5′-GCGGCTTTCCTCACTGGCATG*CA*AGAGAGCCGATACCAACGC-3′
		Antisense: 5′-CCAGGCAAAGAAGCGTTGGTATCGGCTCTC*TT*GCATGCCAGT-3′