Table 3. In vitro α(1,3)-fucosyltransferase activity of FucT-VII and its glycomutants.

Detergent cell extracts were assayed for α(1,3)-fucosyltransferase activity using fetuin as an acceptor. The activities were determined as pmol of fucose transferred·(nmol EGFP)·h⁻¹ (ϵ=55000 M⁻¹·cm⁻¹) (see also Table 2).

	Catalytic activity [pmol·(nmol EGFP)−1·h−1]	Inhibition (%)
Wild-type	5.28±1.12	–
N81Q	0.22±0.10	95.8
N291Q	0.65±0.20	87.7
N81Q/N291Q	0.19±0.14	96.4