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. 2005 Oct 25;391(Pt 3):503–511. doi: 10.1042/BJ20050533

Figure 4. Knock-down of p53 prevents FDH-induced apoptosis in A549 cells.

Figure 4

(A) Apoptosis detected by FACS flow cytometry in Tet-On A549/FDH cells (top panels, +p53) and the same cells with knocked down p53 (bottom panels, −p53) after induction of FDH expression or treatment with Act D. Lower left (double negative), live cells; lower right (annexin V positive, PI negative), early apoptosis; upper right (double positive), late apoptosis; upper left (annexin V negative, PI positive), dead cells. Control, non-treated cells. (B) Percentages of apoptotic cells (sum of early and late apoptotic cells) calculated from (A).