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. 2005 Oct 25;391(Pt 3):503–511. doi: 10.1042/BJ20050533

Figure 6. Accumulation of p53 in nuclei after induction of FDH expression in A549 cells.

Figure 6

Top row, p53 (green) was detected by immunostaining with monoclonal anti-p53 antibodies (clone DO-1, Calbiochem) and polyclonal anti-mouse IgG2a antibodies conjugated with Alexa Fluor 488 dye (Molecular Probes). Middle row, the nuclei (red) were imaged by chromosomal DNA staining with TO-PRO-3 iodide dye (Molecular Probes). Bottom row, overlay with yellow indicating co-localization. Images were obtained by confocal laser microscopy. Time (h) after induction of FDH expression is shown.