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. 2005 Oct 25;391(Pt 3):549–560. doi: 10.1042/BJ20050296

Figure 1. Levels of key rate-limiting enzymes in bile acid synthesis in livers of L-FABP wild-type (+/+) and L-FABP (−/−) mice.

Figure 1

Proteins from liver homogenates of male mice were separated by SDS/PAGE, electroblotted on to nitrocellulose membranes, and incubated with polyclonal antisera directed against CYP7A1 (A), CYP27A1 (B) or SCP-x (C) as described in the Experimental section. The amount of protein is expressed in arbitrary units with that present in the control-fed L-FABP (+/+) liver homogenates defined to be 1. The data represent the means±S.E.M. For L-FABP (−/−) versus L-FABP (+/+): **, P<0.01; ***, P<0.001. For cholesterol diet versus control diet: #, P<0.05; and ###, P<0.001.