Figure 2. Proteins that bind/transport bile acids and the bile acid precursor cholesterol in liver cytoplasm of L-FABP wild-type (+/+) and L-FABP (−/−) mice.

Proteins from liver homogenates of male mice were separated by SDS/PAGE, electroblotted on to nitrocellulose membranes, and incubated with polyclonal antisera directed against cytosolic cholesterol/bile acid transport proteins such as L-FABP (A), SCP-2 (B), caveolin-1 (C), GST (D), or 3α-HSD (E) as described in the Experimental section. The amount of protein was expressed in arbitrary units with that present in the control-fed L-FABP (+/+) liver homogenates defined to be 1. The data represent the means±S.E.M. For L-FABP (−/−) versus L-FABP (+/+): *, P<0.05; **, P<0.01; ***, P<0.001. For cholesterol diet versus control diet: ##, P<0.01; and ###, P<0.001.