Figure 3. Deletion analysis of the 5′-flanking region of the human PiC gene.

The deletion mutants named C1–C8 were cloned into the pEMBL8-CAT plasmid and tested for CAT expression activity in transfected HeLa cells. CAT indicates the pEMBL8-CAT vector alone. Numbering indicates the extent of fragments, whereas dark bars indicate CAT activity. The values were set relative to the C1 construct. Means±S.D. for five duplicate independent experiments are shown.