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. 2005 Oct 25;391(Pt 3):613–621. doi: 10.1042/BJ20050776

Figure 9. Effect of forskolin on PiC promoter activity and on PiC content both at the transcript and protein levels.

Figure 9

(A) Effect of forskolin on PiC promoter activity conferred by the DNA fragment −223/+95 with or without mutation in the CREB binding site. Transfected HeLa cells treated or untreated with 10 μg/ml forskolin were assayed for CAT expression activity. Grey bars indicate HeLa cells transfected with the wild-type (W) DNA fragment −223/+95 and the DNA fragment mutated in the CREB site (M). The black bars indicate forskolin-treated HeLa cells transfected with the constructs W and M. Means±S.D. for three duplicate independent experiments are shown. (B) Effect of forskolin on the PiC mRNA level. RT–PCR was performed with specific primers for the cDNA of PiC or β-actin and with total RNA from HeLa cells treated or untreated with forskolin. (C) Effect of forskolin on the PiC protein content. The PiC protein of HeLa cells treated or untreated with forskolin was immunodecorated with an anti-N-terminal PiC antibody. The reactivity of the antibody to recombinant human PiC-B was also tested.