Figure 6. Ser¹⁸⁴ regulates the level of FoxO6 activity.

FoxO6 wild-type or alanine or aspartic acid mutants of Ser¹⁸⁴ were transfected, together with a six times optimal FoxO-binding site reporter fused to luciferase (6DBE) or the G-6-Pase promoter fused to luciferase (G-6-Pase). Serum application (S) resulted in a decrease in transcriptional activity of the wild-type FoxO6 and Ser¹⁸⁴ mutants. Both 6DBE and G-6-Pase reporters used showed comparable patterns of activity in response to FoxO6 and FoxO6 mutants. The S184A mutant displayed higher activity under serum and serum-free (SF) conditions when compared with wild-type FoxO6, whereas the S184D mutant showed lower activity. The GFP control does not display any transcriptional activity.