Figure 3. Binding of the −152 to −50 bp CYP2J2 promoter region to c-Jun.

The double-stranded probes (A) 2J2-A (−152 to −103 bp of the CYP2J2 promoter), (B) 2J2-B (−127 to −79 bp of the CYP2J2 promoter) and (C), 2J2-C (−102 to −50 bp of the CYP2J2 promoter) were end-labelled with [³²P]dCTP and incubated with recombinant c-Jun. Binding reactions that contained excess unlabelled self probe [2J2-A in (A), 2J2-B in (B) or 2J2-C in (C)], the non-specific Stat probe (Stat5 element from the β-casein promoter) and the smaller overlapping probes 1–9 (refer to Figure 2) are indicated. Retarded complexes are indicated by arrows.