Table 1. Sequences of oligonucleotides used in site-directed mutagenesis to create CYP2J2 5′-truncation and mutation constructs.
Mutated nucleotides are underlined.
| Oligonucleotide | Sense/antisense | Sequence (5′—3′) |
|---|---|---|
| 2J2-mt-(−56/−63) | Sense | GAGGCGGGGCGGGAAAAAAAACCTGCTGGGACCG |
| 2J2-mt-(−56/−63) | Antisense | CGGTCCCAGCAGGTTTTTTTTCCCGCCCCGCCTC |
| 2J2-mt-(−148/−142) | Sense | GCGTGCTAGCCCGCTTAAAAAGCGCCTGGCATC |
| 2J2-mt-(−148/−142) | Antisense | GATGCCAGGCGCTTTTTAAGCGGGCTAGCACGC |
| 2J2-mt-(−141/−134) | Sense | AGCCCGGGAATCCTTTTAAAAGCATCTTCGCAGG |
| 2J2-mt-(−141/−134) | Antisense | CCTGCGAAGATGCTTTTAAAAGGATTCCCGGGCT |
| 2J2-mt-(−133/−126) | Sense | AATCCAGCGCCTGAAAAAAAAGCAGGGTGCTGCG |
| 2J2-mt-(−133/−126) | Antisense | CGCAGCACCCTGCTTTTTTTTCAGGCGCTGGATT |
| 2J2-mt-(−106/−96) | Sense | GGTGCTGCGAAGGGGGAAAAAAAAAAGCGGGGCACGGCTGG |
| 2J2-mt-(−106/−96) | Antisense | CCAGCCGTGCCCCGCTTTTTTTTTTCCCCCTTCGCAGCACC |
| 2J2-mt-(−122/SmaI) | Sense | CTGGCATCTTCGCACCCGGGTGCTGCGAAGG |
| 2J2-mt-(−122/SmaI) | Antisense | CCTTCGCAGCACCCGGGTGCGAAGATGCCAG |
| 2J2-mt-(−82/SmaI) | Sense | CGGGGCACGGCTCCCGGGAGCGAGGCG |
| 2J2-mt-(−82/SmaI) | Antisense | CGCCTCGCTCCCGGGAGCCGTGCCCCG |
| 2J2-mt-(−49/SmaI) | Sense | CCGTCGCCTGCTCCCGGGACCGCCGCC |
| 2J2-mt-(−49/SmaI) | Antisense | GGCGGCGGTCCCGGGAGCAGGCGACGG |