FIG. 4.

AS induction in human plasma is PrgZ dependent. Western blot of cell surface extracts with a polyclonal anti-AS antibody for comparison of AS expression in MSP4301 (prgZ⁺) and MSP4901 (prgZ mutant). Lanes: 1 to 4, MSP4301; 5 to 9, MSP4901; 1 and 6, 10 ng of cCF10 per ml; 2 and 7, 1 ng of cCF10 per ml; 3 and 8, no peptide; 5, 100 ng of cCF10 per ml; 4 and 9, 100% human plasma. Multiple bands are the result of AS degradation. Cell pellets were washed three times before extraction. An equivalent amount of protein was loaded in each lane.