FIG. 5.

Plasma influences the peptide balance. Influence of addition of different ratios of exogenous cCF10 and iCF10 on the frequency of transconjugants per donor in THB (white bars) and human plasma (black bars). The concentration of cCF10 (1), where added, is 10 ng/ml. The concentration of iCF10 added was 20-, 40-, or 60-fold of that of cCF10, respectively. −, no peptides added. Cells were exposed to inducing conditions for 2 h, and mating between the donor and recipient was performed for 10 min (for details, see Materials and Methods).