Skip to main content
. 2005 Oct 25;391(Pt 3):699–710. doi: 10.1042/BJ20050380

Figure 3. JFC1 regulates the secretory pathway of PSA in LNCaP cells.

Figure 3

(A) LNCaP cells were transfected with 10 μg of the pCMV-myc-JFC1 expression vector (pCMV JFC1), the pCMV-myc-C2A (pCMV C2A) or the empty vector pCMV-myc (Empty vector) by nucleofection and were seeded in six-well plates containing RPMI 1640 medium 16 h before treatment. Cells were left untreated (DMSO) or stimulated with 100 nM 6α-fluorotestosterone for 48 h. For each condition, the medium was collected, and the concentration of PSA was analysed using a specific ELISA kit. Results are means±S.E.M. for eight different experiments. The unstimulated empty vector control was arbitrarily designated 100% secretion. The significance of the difference between means was calculated using a one-tail paired Student's t test (InStat 3.0); *P<0.01. (B) PSA co-localizes with overexpressed JFC1. LNCaP cells were transfected with the expression vector pDsRED-JFC1 (upper panel) or with the control vector pDsRED (lower panel), and, 24 h after transfection, the cells were fixed, and the subcellular distribution of endogenous PSA was evaluated by immunofluorescence.