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Isolation of homozygous T-DNA insertion mutant lines carrying the atwrky46, atwrky53 double mutation. (A) Schematic structure of atwrky46 and atwrky53 mutant alleles carrying T-DNA insertions. (B) PCR amplification using gene specific primers shown in panel A. Putative lines homozygous for the atwrky46, atwrky53 double knockout mutations fail to amplify the wild-type allele (marked by circles).
