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. 2005 Aug 23;1:4. doi: 10.1186/1746-4811-1-4

Figure 8.

Figure 8

Sucrose Prep and the 'Touch-and-Go' methods works well in other plant species. DNA was isolated in duplicate using the Sucrose Prep or the 'Touch-and-Go' methods (indicated on the left) from leaves of one month old N. tabacum and N. benthamiana plants (upper two panels) or from leaves of 10 day-old barley plants (lower two panels). PCR was carried out for 40 cycles in a 50 μl reaction volume using primers for NtCDPK2 and NtRBCS in tobacco, and four different pairs of primers for barley. Only 10 μl of the final reaction was resolved by agarose (1%) gel electrophoresis for analysis.