Table 1.
Chr | Marker | Forward primer (5'-3') | Reverse primer (5'-3') | Col-0 (bp) | Ler (bp) |
I | nga63 | GCCTAAACCAAGGCACAGAAG | TCATCAGTATTCGACCCAAG | 87 | 99 |
I | F3F19 | CCACAAAACAATTTGGTTCACTC | TCCCGTTGGGGATATTAAAG | 100, 143 | 243 |
I | F20D23 | TTATGCCAACTCATGTGGAAAG | TGTCAAAGCGTCTGGTTCTG | 233 | 254 |
I | F12K8 | ACCAACACCACAACAAACGAC | CTTTTTCTGTTCTTCCGCTATTC | 171 | 192 |
I | F3I6 | AGATGGAAGAGGAGGAGATGG | TGCATGTATATGATGAGCGAGAG | 251 | 311 |
I | SO392 | GTTGTTGATCGCAGCTTGATAAG | TTTGGAGTTAGACACGGATCTG | 142 | 156 |
I | F7P12 | TCGAGGATATGTTTCGTGTTTG | ACAGTTTTGATGCATTGTGTGAG | 315 | 100, 215 |
I | F1I21 | TCGTAAATTGTGACTGGGAGA | CCCTGTAGATCTGTTGTTTTAG | 308 | 113, 195 |
I | ciw1 | ACATTTTCTCAATCCTTACTC | GAGAGCTTCTTTATTTGTGAT | 159 | 135 |
I | nga280 | CCTGATCTCACGGACAATAGTG | GGCTCCATAAAAAGTGCACC | 106 | 86 |
I | F23H11 | GATATGGGAGTAAGTATGAAATCGG | TTCGTCCGGGTAAAAGTCAAG | 300 | 250 |
I | NF514a | GTTGAGTCTTGGCATCACAGTTC | CTGCCTGAAATTGTCGAAAC | 221 | 240 |
I | F20P5 | GATACGTTCAAAATTAGGGACTTC | TGTATTTTGCTAATTGAGGTTATGG | 218 | 186 |
I | AthATPase | CCTGGGAACGGTTCGATTCGAG | GTTCACAGAGAGACTCATAAACCA | 86 | 70 |
II | T20F6 | CGTTCGAAACTGAATTAGCTG | ACCATCTTTGTTGAGCCCTTC | 297 | 347 |
II | F18P14 | ATTCCCGCAATTTATTTTGTTC | GTTTGATGGCAGATTTGTTTTC | 123 | 144 |
II | ciw3 | GGAAACTCAATGAAATCCACTT | GTGAACTTGTTGTGAGCTTTGA | 230 | 200 |
II | F26B6 | CTCTATCTGCCCACGAACAAG | GCCATTGCAAAAGAACATCAG | 233 | 274 |
II | F16P2 | CAGCAATCAAATAACGTGGTG | CTCTCTTCTTTCTTCGCCATTAG | 237 | 167 |
II | F4P9 | TGGTCCATACCCATTTCATAAC | ATGAATTTTCATTCTACTGTTTTG | 299 | 262 |
II | T2H17 | ATTGCATACCACGCAGTTCAC | CCATTTTGCCCTTTCCTTCTAC | 250 | 274 |
II | AthBIO2b | TGACCTCCTCTTCCATGGAG | TTAACAGAAACCCAAAGCTTTC | 141 | 209 |
III | nga172 | AGCTGCTTCCTTATAGCGTCC | CCATCCGAATGCCATTGTTC | 162 | 136 |
III | nga162 | CATGCAATTTGCATCTGAGG | CTCTGTCACTCTTTTCCTCTGG | 107 | 89 |
III | MSA6 | TTGGAGGTGCTCTTAGGTTC | GGGCTTTTCACATACGCTTTC | 175 | 225 |
III | ciw11a | GTTTTTTCTAATCCCCGAGTTGAG | GAAGAAATTCCTAAAGCATTC | 192 | 242 |
III | N7N14 | CAATACACTTTATCCAGATGCTG | GGGATTTGTTGATTGAAAAAGGAC | 150 | 143 |
III | T6H20 | CGGCTGAAACTTGGAAGGGAC | AGGAAGAACGTGTGATTGTG | 273 | 293 |
III | ciw4 | GTTCATTAAACTTGCGTGTGT | TACGGTCAGATTGAGTGATTC | 189 | 215 |
III | K27K19 | TGCTTTTGAAGAGATGGTTATTAGG | CCCCATTTCACTTATCATTGG | 216 | 198 |
III | nga6a | AGCGAATCCGAAAATAATGGAG | TGGATTTCTTCCTCTCTTCAC | 159 | 137 |
IV | ciw5 | GGTTAAAAATTAGGGTTACGA | AGATTTACGTGGAAGCAAT | 164 | 144 |
IV | F14G16 | ACAAACCGATCAGCATTCAAG | GCCTTTGTCACGGATTCAAC | 250 | 198 |
IV | T3H13 | TTTGGTGGGTCAAGAGTCAAG | GCAAAAGTCATTACGGACAATAC | 275 | 229 |
IV | T26M18 | CAATTAGCGGAGGCCACTTC | GGGCAAAAGCTTCCAGTAC | 330 | 271 |
IV | FCALL | CCACCGTCAACATCCCTAAC | GCTCTTATACTTCTCAGCTCTTGTC | 170 | 180 |
IV | F28A21 | GCATCATCATTCATCACCAAC | TGTGAAGTGTTTGTCTTTGTG | 198 | 169 |
IV | F16G20 | TGTCAACCAATCGCCTTAGTC | TTAATGTCCATTATTGGAACGC | 113 | 79 |
IV | F26K10 | AGAGAGCACGATGCCTGATAG | AATGCTTCAGCGATTGAGAAC | 180 | 205 |
IV | F6E21 | TTCTTTGTTCAAGTTCCATGTCTC | CGGTGATTGTCTCAAGTGTTTG | 199 | 225 |
IV | F23E13 | TGACCGTTGAAAGTGTTGTTG | GCCCGAGAAGCCTGATAG | 264 | 246 |
V | MHF15 | CTCCTCCTTTAATTTTCTCTCTGTG | AGTTCCAGCTTTGGACTTCTTC | 295 | 268 |
V | nga151a | ATCTCATACTGACCCATATGTTCC | ATTGTACAGTCTAAAAGCGAGAG | 198 | 170 |
V | ciw8a | TACTAGTGAAACCTTTCTCAG | TTTTATGTTTTCTTCAATCAGTTAG | 100 | 135 |
V | nga139 | AGGGTTTCGTTTCACTATCCAG | TGAGAGCTACCAGATCCGATG | 174 | 132 |
V | T1N24 | CCGATGGCATAACAAGTAGAG | GGGAAAGGTACACATATACAAAAGG | 383 | 356 |
V | nga76 | GGAGAAAATGTCACTCTCCAC | AGGCATGGGAGACATTTACG | 231 | 250 |
V | ciw9 | CAGACGTATCAAATGACAAATG | GACTACTGCTCAAACTATTCGG | 165 | 145 |
V | MPL12 | GTCCCCAAAACCAATCATAAG | TCCGAGTGAGAAGAGAGTTTG | 319 | 293 |
V | K9P8 | TTATGGGTTTCTCAGAGTTTCTCAC | TTGTATGCGTTTGCTTTTTCC | 284 | 251 |
V | MNC17 | GTACCGGATCTGTGTTGTGAAG | GTGCTCAAGGAAATGGGATAG | 168 | 187 |
V | MQB2 | CTTTGATAGTAACCTTTTTCAAACCA | TGCCATTTATTTGGTCAACAC | 252 | 231 |
DNA oligonucleotide primer pairs are listed according to their positions from the top to the bottom of each chromosome. The size (bp) of PCR amplification products from Col-0 and Ler are depicted in the last two columns to the right. F, forward primer and R, reverse primer.