TABLE 1.
Bacterial strains and plasmids used in this study
| Strain and plasmid | Relevant characteristics | Reference |
|---|---|---|
| S. pyogenes strains | ||
| SSI-9 | M type 1, isolated from patient with TSLS | T. Murai and Y. Shimizu |
| SSI-1 | M type 3, isolated from patient with TSLS | T. Murai and Y. Shimizu |
| #42 | M type 12, isolated from patient with TSLS | H. Watanabe |
| TR-7 | Isogenic mutant of SSI-9; derivative of pYT1088; lbp::aphA3 Kmr | This study |
| SE strains | Clinical isolates in Japan | Saga Prefectural Institute of Public Health |
| MJ strains | Clinical isolates in Japan | Osaka Prefectural Institute of Public Health |
| Others | Laboratory collection | |
| Other streptococcal strains | ATCCa | |
| Plasmids | ||
| pGEX-6P-1 | Expression vector; Ampr | Amersham Pharmacia Biotech |
| pSF151 | Suicide vector for insertional mutagenesis; Kmr | 27 |
| pYT1088 | pSF151 with lbp from SSI-9; Kmr | This study |
| pYT1097 | pGEX-6P-1 with lbp from SSI-9; Ampr | This study |
a
ATCC, American Type Culture Collection.