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. 2005 Oct 4;1:7. doi: 10.1186/1746-4811-1-7

Figure 4.

Figure 4

Confocal and biparametric flow cytometric analyses of wild-type and transgenic plants expressing nuclear GFP. Flow cytometry was done using a Cytomation MoFlo flow cytometer with laser excitation at 365/488 nm, and biparametric detection of DAPI fluorescence (418–482 nm; FL4; log units), and GFP fluorescence (505–530 nm; FL1; log units), with triggering based on 90°-light scatter [59]. For confocal microscopy, roots were counterstained by dipping in propidium iodide (1 μg/mL in water) for 2 minutes. Abbreviations: p35S: CaMV 35S promoter; pSCR: SCARECROW promoter; pSHR: SHORTROOT promoter; pRPL16B: ribosomal protein large subunit 16B promoter; pSultr2-1: sulfate transporter 2-1 promoter.