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Microscopic evaluation of wild-type and CSP knockout P. knowlesi oocyst development in A. stephensi midguts. (A) Intact wild-type oocyst 9 days postfeeding. (B) Intact CSP knockout oocyst 11 days postfeeding. (C) Wild-type oocyst releasing sporozoites (marked with arrows) 8 days postfeeding. (D) Force-induced rupture of CSP knockout oocyst 11 days postfeeding. Sporozoite release was never observed in CSP knockout oocysts.
