FIG. 5.

Transferrin binding by L5 and L4-L5 in a solid-phase chemiluminescent binding assay. Dialyzed inclusion body preparations were probed with either HRP-Tf (solid line) or HRP-Tf in the presence of excess saturated transferrin as a competitor (dashed line). The results are plotted as relative luminescence units (RLU) as a function of the concentration of HRP-Tf added. The individual panels represent total binding to the fusion proteins L5 (A), L4-L5 (B), plug (C), L2 (D), L3 (E), and L9-L10 (F), CBD alone (G), and full-length rTbpA (H). Error bars represent standard deviations.