TABLE 3.
Presentation of peptides p4 and p9 by class II major histocompatibility complex I-Ad haplotype on APC to M. leprae ESAT-6-specific-T-cell hybridomas
| Hybridoma | Peptide | Results with APCb
|
||
|---|---|---|---|---|
| A20 (I-Ad I-Ed) | M12.B5 (I-Ad) | M12.A2 (I-Ed) | ||
| 2A3 | p4 | +a | + | - |
| 3D8 | p9 | + | + | - |
| 6B7 | p9 | + | + | - |
a
Positive responses indicate IL-2 production as detected by cytokine ELISA. Optimal stimulation of T-cell hybridomas with 1 μg of peptide induced >500 pg of IL-2/ml, while no peptide or inappropriate peptide controls gave background levels corresponding to <20 pg of IL-2/ml (−).
b
APC, antigen-presenting cells.