TABLE 1.
Translocation and tyrosine phosphorylation of CagA, induction of the scattering phenotype, and secretion of IL-8 in AGS cells infected with H. pyloria
| Bacterial strain | Mutated ORFb | CagA expres- sion | CagA trans- location/phos- phorylation | Induction of scattering phenotype | Induction of IL-8 secretion |
|---|---|---|---|---|---|
| P1 wt | + | + | + | + | |
| P12 wt | + | + | + | + | |
| G27 wt | + | + | + | + | |
| J99 wt | + | − | − | + | |
| P12ΔvacA | HP0887 | + | + | + | + |
| P1ΔalpA/B | HP0912/HP0913 | + | − | − | − |
| P12Δcag PA1 | HP520-HP547 | − | − | − | − |
| P1ΔvirB4 | HP0544 | + | − | − | − |
| P1ΔvirB7 | HP0532 | + | − | − | − |
| P1ΔvirB8 | HP0529 | + | − | − | − |
| P1ΔvirB9 | HP0528 | + | − | − | − |
| P1ΔvirB10 | HP0527 | + | − | − | − |
| P1ΔvirB11 | HP0525 | + | − | − | − |
| P1ΔvirD4 | HP0524 | + | − | − | + |
| P1ΔcagA | HP0547 | − | − | − | + |
| G27ΔcagF | HP0543 | + | − | − | − |
| P1ΔcagH | HP0541 | + | − | − | − |
| G27ΔcagI | HP0540 | + | − | − | − |
| G27ΔcagM | HP0537 | + | − | − | − |
| P1ΔcagA/cagA | HP0547 | + | + | + | + |
| P1ΔvirD4/virD4 | HP0524 | + | + | + | + |
a
All H. pylori strains are type I isolates. cag PA1 mutagenesis and genetic complementation are described in Materials and Methods (see also Fig. 1). Bacterial infection was for 6h at an MOI of 100. CagA translation and tyrosine phosphorylation were detected by immunoblot analysis. IL-8 production was determined by ELISA. wt, wild type
b
From Tomb et al. (39).