TABLE 2.
Bacterial numbers in organs of naive and sensitized mice after vaccination and aerosol challenge with virulent M. tuberculosis
| Vaccine groupa | Result in:
|
||||
|---|---|---|---|---|---|
| Lung
|
Spleen
|
||||
| Log10 CFUb | Log10 resistancec | Log10 CFU | Log10 resistance | ||
| Expt 1 | |||||
| Naive | |||||
| Control | 6.36 ± 0.08 | 4.71 ± 0.05 | |||
| BCG | 5.83 ± 0.06 | 0.53* | 4.12 ± 0.12 | 0.59* | |
| DDA-MPL | 6.34 ± 0.09 | 4.94 ± 0.12 | |||
| ESAT-6 | 5.76 ± 0.09 | 0.60* | 4.39 ± 0.09 | 0.32* | |
| Sensitized | |||||
| Control | 6.18 ± 0.08 | 4.82 ± 0.16 | |||
| BCG | 6.27 ± 0.07 | <0.05 | 4.79 ± 0.05 | <0.05 | |
| DDA-MPL | 6.39 ± 0.05 | 4.73 ± 0.11 | |||
| ESAT-6 | 5.74 ± 0.16 | 0.44* | 4.43 ± 0.05 | 0.39 | |
| Expt 2 | |||||
| Naive | |||||
| Control | 6.88 ± 0.12 | 5.10 ± 0.18 | |||
| DDA-MPL | 7.19 ± 0.05 | 5.48 ± 0.11 | |||
| Ag85B-ESAT-6 | 6.03 ± 0.12 | 0.85* | 4.40 ± 0.08 | 0.70* | |
| Sensitized | |||||
| Control | 6.30 ± 0.08 | 4.39 ± 0.09 | |||
| DDA-MPL | 6.49 ± 0.05 | 4.27 ± 0.08 | |||
| Ag85B-ESAT-6 | 5.37 ± 0.14 | 0.93* | 3.89 ± 0.07 | 0.50* | |
a
Naive or sensitized mice were immunized s.c. with BCG or injected three times with a subunit vaccine emulsified in DDA-MPL.
b
Bacterial numbers are given as log10 CFU of M. tuberculosis isolated from the lung and spleen 6 weeks after aerosol challenge with virulent M. tuberculosis.
c
Protective effects of the two vaccines are expressed as log10 reductions in bacterial numbers compared to those in unvaccinated control mice. Bacterial numbers significantly different from those seen in control mice are indicated by an asterisk.