FIG. 1.

Increased transcription of the Mga regulon in NZ131 rgg. Total RNA was isolated from exponential-phase cultures of wild-type (wt) NZ131, speB mutant strain NZ131, and rgg mutant strain NZ131, and the quantities of gene-specific transcripts were determined by quantitative RT-PCR. The quantity of cDNA for each gene was normalized to the quantity of gyrA cDNA in each RNA sample. The values shown are the mean ± the standard error of the mean of at least two independently isolated RNA preparations analyzed in triplicate.