TABLE 1.
Oligonucleotide primers and fluorescent probes used for TaqMan RT-PCR
| Accession no. | Designationa | Forward primer (5′-3′) | Reverse primer (5′-3′) | Fluorescent probeb (5′-3′) |
|---|---|---|---|---|
| AF057697 | sof49 (SOF) | CACTACAAAGACGATTGGTGCTAAA | CAACACCCCAGTCCCAATCT | TAGAGGAAGTTTGGGAGCAGGCCAAAA |
| M31789 | emm49 (M49) | GCATCCGTAGCGGTCGC | CAGCAGCCTTAACTTCTGTTTGG | TGGCTGTTTTAGGAGCAGGCTTTGCA |
| X68501 | mga49 (Mga) | GCTCAATCTCAGCATCACCAATAA | AATGTCTTCTTCGTCTAACACCGA | ACGAAGTTGCCGTAACCCTCCATAACG |
| X72753 | enn (Enn) | AGCACCAAAAATTAGAGGCTGATC | GATGCTTCAAGGTCACGGCT | CCAAGTTTCAGAGACTAGCCGCAAGGG |
| X78055 | scpA49 (C5a peptidase) | AAACGAAAGAACCTTACCGCC | CGACACGCATCAAAAGCAA | AGAAGGTGCGATGCCTGAGGCTCA |
| X80397 | orfX (OrfX) | TCTACTGAGTCTACTACTCAGCCAGTTGA | TGTCACCATCGAATCTGAAGCT | CACCCGTGCAGGAGACACAGGC |
| SPy0167 | slo (SLO) | GAAATATCCGTATCATGGCTAGAGAGT | CACATCTCTTTCGTCGATCACTTT | GCCACCATTCCCAAGCTAAGCCAGTG |
| SpyO212 | speG (SpeG) | TTCTATTGTATCTTTAGGCATTACTGATCA | TTCGAATGTTTTACAATACCACCATAA | CGATGTTTTTGGTCTCCCTTATAATTTTTCCCC |
| SPy0242 | fasB (FasB) | TGAGCCGTATAGAGCGTTTAGGT | TCATGAACCGTTTGGGCATA | AAGCCATTAAGAGTGAATCCATTACTCAGATTCAAGA |
| SPy0244 | fasC (FasC) | CACAAAAACCAATCGTGTGGATA | CAGTCAAAAAGTGGGCTGAGTTC | AGCCAAACGCTTTGATGCTCAGCAG |
| SPy0245 | fasA (FasA) | CGTTGTGGGCTTGAATTAGCT | ATTCTGAATGAGTGGTGACAAACAC | CAGCTATTCGACAAAAAGATCCTAATGCCGTTAT |
| SPy0336 | csrR (CsrR) | GGTGCAGACGACTATATTGTTAAACCC | TTGACGGCGGAAAATAGCA | TTTGCCATTGAAGAACTACTTGCCCGTATTC |
| SPy0337 | csrS (CsrS) | CCTGGCTTGCATGGTCCTA | CATGGAAAACCCACGATACTGA | CGGTCGTGTGTATCACGACCATATCGA |
| SPy0528 | vicR (VicR) | ACGTGACCAAGCCCTTTTCTAA | TTCGGTACGACGCAGATGAG | CACGCGCCAGCAATTCCCG |
| SPy0529 | vicK (VicK) | GCGCTTTGCGTTGAATAGG | TCCTGTTCTGTGGTGTCATGGA | CCACAACCAAGCCCGAAATAAAACCACT |
| SPy0738 | sagA (SLS) | TTGCTCCTGGAGGCTGCT | CTTCCGCTACCACCTTGAGAAT | ACCACTTCCAGTAGCAATTGAGAAGCAACAAG |
| SPy0747 | None (none) | TTGTGTGCAACCAGTCACTTTTAA | TCTTTCGCAAATTGTGCTAGCA | TCTGAGCAAAGACGTCACGTCTTGGCTA |
| SPy0861 | mac (Mac) | ACTTCCGTTTGGACCAAAGGA | CAACATAAGGAGCGTGAAAAACATC | TTACTCCTCCAGCAAACTTCACTCAAGGTGA |
| SPy0874 | trcR (TrcR) | TGGCAGATGTCTAATGTTTTGACA | TGCCTCTTGACCGTTAAAAACC | CTGCCATGACTCATCAAGGTTATGACGTTG |
| SPy0875 | None (none) | TTCCGGTATGGTCATCAAACC | ACTCGTGGCCTGCATTAGTAATAA | GCGACGCTGCTTCTCAGAATTTTGGACA |
| SPy0895 | deoD2 (DeoD2) | TTGCCTTTCTAGCGGAACATG | GCGATTGGTTAAGCCATAATGAAC | CAGAGAATCGGACCATTGAAGACCAGTATCAC |
| SPy0898 | cpsY (CpsY) | CACAACTCCTTTTATTTTTCAGAAGAGA | GCACGGTCACTGACCACAAT | ATGTCCCAGATGCCCCACAACAAATC |
| SPy1008 | speH (SpeH) | TCACAGGAATTCAAAGATAAAGAAGTAGA | CAAACGCTTCATACCTTTTCCC | ATTTATGCTCTATCTGCACAAGAGGTTTGTGAATGT |
| SPy1061 | yesN (YesN) | GTTATCATTGTTGCTAATTGGTCTTTTTAT | GGTTGCAGTGGCATTGATATCA | TCATTAGCGCCACAACAATTAGGCCTCTATC |
| SPy1062 | yesM (YesM) | TTGGAGTGGGTACATGCTCATT | AACTCTCACTATACCCTAAGTCATGAGCTA | TCAAGACAAGATCAGCACGAGCCGC |
| SPy1106 | yufM (YufM) | GCAATGGGATTCAATTTTTGGA | ATTTGCGGCTGAAATAATAATGACT | AAATGGCGTACTCAGCATATTCCTTGCG |
| SPy1236 | ciaS (CiaS) | GGTCCAGGGATAACAGATGAAGAA | CCACCTGTTTGCCGTGTTC | AGCTTGTCAACTCGATAAAAACGATCAAAAATCTTT |
| SPy1237 | ciaR (CiaR) | GATCATGACAGCTAAAGAGGGTTTAGA | AATAGAACGGCTTTGTCAGATAATCA | CAGCACCTAATTCAAATCCGTGGCCTT |
| SPy1357 | grab (Grab) | GCATCAGTATTAGTCGGTTCAACAGT | CCGCCATTTGGAATAATTCG | TCTGCTGTTGACTCACCTATCGAACAGCC |
| SPy1436 | mf-3 (MF-3) | TCCTCCTGGCTGGCATAATT | CTAAATGGCCACGGTCCATT | AAATTGACTGACGCTAATGGAAAAACAACTTGG |
| SPy1556 | zmpS (ZmpS) | AAAAGAAGTATCAGCAATAGCAATGG | TGCTGAGCCAAGCTTTTTAGG | TCAAATCAGAATCTGCCAACCGCTCA |
| SPy1587 | lytR (LytR) | GATTTTACAAGGGCTCACCATGA | TGCCTGATCTTGCTGTTTGAA | ACTTCAAATCCAAGTGCCTGCCAATCA |
| SPy1588 | lytS (LytS) | TTTACCTCCGTCTCTTTCATCATTT | AAGCCACCATTTGGCTCCTT | CGAGACAGGGTTTCTTGGTCCATGGC |
| SPy1801 | isp-2 (Isp-2) | CGTCGCGCACAAATTATGG | GGCTGGAATGGCTGGTGT | CCATGGTCATCAATCCACTTATCCAGGTCT |
| SPy1908 | salR (SalR) | TTGATGACCACAGACTATTTGCAA | ACATCGTTAAAATGAGAAGTAATCGTATCT | CCAACTGTTATTCCAACAGTATGATGAAGTAGATGTCA |
| SPy1909 | salK (SalK) | GGATTGTACTCATGAAGGTATTAGTTAAAGA | TTGGAGTTTTCTAAATAATAAAAGATGCA | GAAATTACCACTAGACAAAACAACGTGCTTGCGT |
| SPy1979 | ska (SKA) | TGCTGACAAAGATGGTTCGG | GCACATGTCCCTTTAACAAAAATTC | ACCTTGCCGACCCAACCTGTCCA |
| SPy1983 | scl1 (Scl-1) | CAAACTAGTCTGGCGCTACGG | TTGCCCCGCCTCCAA | CCTCAGCCGCTGCCGTCCTTCT |
| SPy2025 | isp-1 (Isp-1) | GCAGCGCAAGCCAGGTT | CTTGGCCTGGTGCAAATGA | ACCACCCATAAACCTAAAGTGGGCTA |
| SPy2026 | ihk (Ihk) | GGTGACTTTGCAGATCGTGACA | TTGAACCAGTTGGGCTTGC | AAGGACGTCTCGGCACTTTCGCAAATAT |
| SPy2027 | irr (Irr) | TCGAAAATGTATTACGCGTCTCTACT | CGCTATGTTCGGTCTCATCAAC | CAGATGAAAAGCGCCAAATCGGAGACTTAC |
| SPy2043 | mf-1 (MF-1) | CCCAAAATGTAGGAGGTCGTG | CATTCTTGAGCTCTTTGTTCGGT | CCAAAAAGGCGGCATGCGCT |
| SPy2200 | hasA (HasA) | ACCGTTCCCTTGTCAATAAAGG | CGTCAGCGTCAGATCTTTCAAA | CGCCATGCTCAAGCGTGGGC |
| SPy2201 | hasB (HasB) | GAATCGAGAAAATTAAATAGTCACATGATT | CCTCCATAACCAAATGATGGGT | TTCAAGGAATTTCTTATGATGATCGAATAGGAATGC |
| SPy2202 | hasC (HasC) | TGAAACCACTGCCATTAACCTTC | GCTTGTAAGACAGCATCTCCCA | TTTTATCCGTCAAAGCCACCCAAGAGG |
a
Gene designations are shown in italics, and the corresponding protein designations are in parentheses.
b
Covalently linked at the 5′ end to 5-carboxyfluorescein and at the 3′ end to N,N,N-tetramethyl-6-carboxyrhodamine.