TABLE 4.
Quantitative RT-PCR analysis of virulence-associated gene transcripts
| SPy no. | Designationb | Quantity of transcripta (mean ± SEM)
|
rgg/wild-type ratioc | ||
|---|---|---|---|---|---|
| Wild-type NZ131 | speB mutant NZ131 | rgg mutant NZ131 | |||
| 0167d | slo(SLO) | 0.09 ± 0.0 | 0.10 ± 0.0 | 1.25 ± 0.1 | 13.9 |
| 0212 | speG (SpeG) | 0.42 ± 0.1 | 0.56 ± 0.0 | 0.33 ± 0.0 | 0.8 |
| 0747 | None (none) | 0.31 ± 0.0 | 0.26 ± 0.0 | 1.00 ± 0.1 | 3.2 |
| 0861 | mac(Mac) | 0.03 ± 0.0 | 0.04 ± 0.0 | 0.06 ± 0.0 | 2.0 |
| 1008 | speH(SpeH) | 0.090 ± 0.0 | 0.11 ± 0.1 | 0.18 ± 0.1 | 2.0 |
| 1357 | grab(Grab) | 1.80 ± 0.2 | 1.71 ± 0.3 | 0.20 ± 0.1 | 0.1 |
| 1436 | mf-3(MF-3) | 0.14 ± 0.0 | 0.14 ± 0.0 | 3.47 ± 0.0 | 24.8 |
| 1801 | isp-2 (Isp-2) | 3.91 ± 0.9 | 3.36 ± 0.9 | 3.63 ± 0.8 | 0.9 |
| 2025 | isp-1(Isp-1) | 1.07 ± 0.3 | 0.63 ± 0.1 | 0.46 ± 0.1 | 0.4 |
a
The gene-specific cDNA values were normalized to the quantity of gyrA cDNA in each sample. The mean ± the standard error of the mean of two independently isolated RNA samples is given.
b
Gene designations are in italics, and the corresponding protein designations are in parentheses.
c
Quantity of gyrA-normalized cDNA in rgg mutant NZ131 ÷ quantity in wild-type NZ131.
d
Boldface denotes differences in transcripts between wild-type NZ131 and rgg mutant NZ131 of less than 0.5 or greater than 1.5.