TABLE 5.

Quantitative RT-PCR analysis of selected regulatory gene transcripts

SPy no.	Designationb	Quantity of transcripta (mean ± SEM)			rgg/wild-type ratioc
		Wild-type NZ131	speB mutant NZ131	rgg mutant NZ131
0242d	fasB(FasB)	0.50 ± 0.1	0.55 ± 0.0	0.23 ± 0.0	0.5
0244	fasC(FasC)	0.69 ± 0.1	0.68 ± 0.1	0.28 ± 0.0	0.4
0245	fasA(FasA)	0.28 ± 0.0	0.29 ± 0.0	0.15 ± 0.0	0.5
0528	sycF (SycF)	1.74 ± 0.2	3.55 ± 0.1	2.08 ± 0.0	1.2
0529	sycG (SycG)	1.36 ± 0.2	1.65 ± 0.1	1.31 ± 0.2	1.0
0874	trcR (TrcR)ef	1.21 ± 0.2	1.36 ± 0.0	1.70 ± 0.2	1.4
0875	None (none)	1.25 ± 0.0	1.31 ± 0.2	2.28 ± 0.6	1.8
0895	cpsX(CpsX)	0.92 ± 0.1	1.16 ± 0.1	1.80 ± 0.2	2.0
0898	cpsY (CpsY)	0.34 ± 0.0	0.33 ± 0.0	0.35 ± 0.0	1.0
1061	yesN (YesN)	0.67 ± 0.1	0.46 ± 0.0	0.59 ± 0.2	0.9
1062	yesM (YesM)	0.39 ± 0.0	0.34 ± 0.1	0.34 ± 0.0	0.9
1106	yufM(YufM)eg	0.28 ± 0.1	0.39 ± 0.0	0.53 ± 0.0	1.9
1236	ciaS (CiaS)eh	1.33 ± 0.4	1.49 ± 0.4	1.15 ± 0.2	0.9
1237	ciaR (CiaR)eh	0.73 ± 0.2	0.92 ± 0.1	0.81 ± 0.2	1.1
1556	zmpS (ZmpS)eh	0.48 ± 0.1	0.44 ± 0.1	0.55 ± 0.1	1.1
1587	lytR(LytR)ei	0.21 ± 0.0	0.15 ± 0.0	0.41 ± 0.1	2.0
1588	lytS (LytS)ei	13.4 ± 2.2	21.7 ± 7.9	16.0 ± 0.9	1.2
1908	salR (SalR)ej	0.58 ± 0.0	0.57 ± 0.1	0.58 ± 0.19	1.0
1909	salK (SalK)ej	2.95 ± 0.5	3.35 ± 0.1	2.92 ± 0.6	1.0
2026	ihk(Ihk)	1.23 ± 0.2	1.18 ± 0.1	0.69 ± 0.0	0.6
2027	irr(Irr)	0.71 ± 0.2	0.64 ± 0.1	0.33 ± 0.0	0.5

^aThe gene-specific cDNA values were normalized to the quantity of gyrA cDNA in each sample. The mean ± the standard error of the mean of two independently isolated RNA samples is given.

^bGene designations are in italics, and the corresponding protein designation are in parentheses.

^cQuantity of gyrA-normalized cDNA in rgg mutant NZ131 ÷ quantity in wild-type NZ131.

^dBoldface denotes differences in transcripts between wild-type NZ131 and rgg mutant NZ131 of less than 0.5 or greater than 1.5.

^eDesignation based on homology with a similar gene in indicated bacterium.

^fMycobacterium tuberculosis.

^gBacillus subtilis.

^hStreptococcus pneumoniae.

ⁱStaphylococcus aureus.

^jStreptococcus salivarius.