FIG. 6.

Immunohistochemical detection of LL-37/hCAP18 in human colon xenografts. (A) Total DNA isolated from luminal contents of two different human fetal colon xenografts (XG1 and XG2) was amplified by PCR using universal primers targeting a highly conserved region of the bacterial 16S rRNA gene. In the lanes designated “XG1 + E. coli 10³” and “XG2 + S. bovis 10³,” the indicated bacteria (10³ CFU) were mixed with xenograft luminal content before DNA extraction. DNA samples from mouse feces and human colon lavage fluid were analyzed as positive controls. (B and C) Human colon xenografts were immunostained with LL-37/hCAP18-specific antibody (B) or control rabbit IgG (C). Magnification for both panels, ×400.