FIG. 4.

CneF-injected sponges in mice immunized with the protective immunogen, CneF-CFA, contained significantly higher levels of TNF-α mRNA than CneF-injected sponges in mice immunized with the nonprotective immunogen, HKC-CFA. The experimental design was similar to that described in the Fig. 1 legend. The sponges were removed 18 h after injection of antigen or saline, and RNA was extracted as described in Materials and Methods. The data are expressed in the same manner as described in the Fig. 3A legend. Where there are significant differences, bars indicate the groups compared. The P values are shown above the bars.