TABLE 1.

Biacore study of the binding of Sh28GST protein and peptides of Sh28GST to immobilized testosterone^a

Protein	Plateau value (RU)	Ka (1/Ms)	Kd (1/s)	Kd (M)
Sh28GST	50	3.28 × 103	1.87 × 10−3	5.7 × 10−7
Peptide 24–43	81	44	3.25 × 10−4	7.4 × 10−6
Peptide 115–131	5	ND	ND	ND
Peptide 190–211	14	ND	ND	ND

^aValues correspond to peptide concentrations of 100 μg/ml and to an Sh28GST concentration of 10 μg/ml. ND, not determined.