FIG. 2.
Release of β-glucuronidase is triggered by aggregates of M. smegmatis. (A) Neutrophils were incubated with 6-day-old unicellular mycobacteria (bar 1), small aggregates of mycobacteria (bar 2), or unicellular mycobacteria obtained by disaggregating small aggregates with glass beads (bar 3). The release of β-glucuronidase was measured, and the results are expressed as means + SDs (error bars) of three experiments. Stimulation of β-glucuronidase exocytosis triggered by OZ is also shown. (B) Neutrophils were incubated with 6-day-old unicellular mycobacteria (bars with oblique lines) or small aggregates of mycobacteria (bars with vertical lines) at the indicated multiplicity of infection (MOI). The release of β-glucuronidase was measured, and the results are expressed as means + SDs (error bars) of two experiments performed in triplicate. Statistically significant difference versus control: ∗, P < 0.05; ∗∗∗, P < 0.001.