FIG. 2.

Hemolytic and IFN-γ-inducing activities of purified LLO. (A) LLO (500 nM) was treated without (open circle) or with cholesterol (10 μg/ml; solid circle) or with heating at 94°C for 15 min (open square). The samples were diluted and mixed with 1% SRBC. The suspension was incubated for 30 min, and the percent release of hemoglobin (Hb) was measured. (B) Spleen cells (10⁶ cells) were stimulated with LLO samples which had been treated as described above. The level of IFN-γ production was determined by ELISA. Shown is a representative result of three similar experiments. Data are means ± standard errors for three determinations.