FIG. 4.

Kinetics of IL-1β (A and B) and RANTES (C and D) mRNA expression in macrophages after M. tuberculosis H37Rv infection. Macrophages from naïve and BCG-vaccinated guinea pigs were infected in vitro with M. tuberculosis H37Rv at an MOI of 1:30 for specific periods of time. At regular intervals, adherent cells from the cultures were used for RNA analysis. The results are representative of the results of RNA blot analyses (A and C) and densitometric analyses (B and D) in three separate experiments. When the naïve and BCG-vaccinated groups were compared, the P value was <0.0001, as determined by ANOVA. A dagger indicates that the P value for a comparison of uninfected macrophages and an infected culture was <0.05, as determined by Dunnett's test.