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Hemolytic properties of LukF-PV and HlgB mutants compared to wt controls. (A) Hemolytic activity on RRBC, expressed as 1/critical concentration (nanomolar−1) of the active LukF-PV mutants associated with either HlgA (black) or HlgC (white). Only LukF-PV mutants ΔS125-L128, ΔG127-S129, and ΔN123-G127 are hemolytic with HlgC. (B) Hemolytic activity on RRBC (black) and on HRBC (grey) of HlgB wt and HlgB G130D in association with HlgA.
