TABLE 1.
Binding of the mutated LukF-PV and HlgB
| Class F protein | Class S protein binding ability (nM)
|
||||
|---|---|---|---|---|---|
| LukS-PV
|
HlgA IC50 | HlgC IC50 | |||
| IC50a | Ki(app)a | ||||
| LukF-PV | 21.1 ± 6 | 2.4 ± 0.7 | 27.6 ± 8 | 37.9 ± 9 | |
| LukF-PV Gly130Asp | 5.5 ± 0.8 | 0.5 ± 0.1 | 2.1 ± 0.4 | 6.9 ± 1 | |
| LukF-PV Gly131Asp | 21.5 ± 5 | 2.4 ± 0.6 | 28.2 ± 4 | 40.3 ± 8 | |
| LukF-PV Ser129Ala | 22.4 ± 4 | 2.6 ± 0.5 | 30.1 ± 7 | 40.9 ± 8 | |
| LukF-PV ΔAsn123-Gly127 | 9.8 ± 2 | 1.1 ± 0.2 | 12.9 ± 5 | 19.3 ± 4 | |
| LukF-PV ΔIle124-Asn126 | 10.8 ± 2 | 1.2 ± 0.2 | 13.5 ± 5 | 16.1 ± 5 | |
| LukF-PV ΔIle124-Ser129 | 62.6 ± 8 | 6.9 ± 0.9 | 79.7 ± 12 | 88.4 ± 11 | |
| LukF-PV ΔSer125-Gly127 | 19.1 ± 4 | 2.1 ± 0.4 | 26.1 ± 7 | 30.9 ± 5 | |
| LukF-PV ΔSer125-Leu128 | 16.1 ± 7 | 1.8 ± 0.8 | 22.1 ± 9 | 28.3 ± 8 | |
| LukF-PV ΔGly127-Ser129 | 22.6 ± 6 | 2.5 ± 0.7 | 26.4 ± 6 | 33.7 ± 6 | |
| HlgB | 4.9 ± 0.5 | 0.4 ± 0.1 | 4.5 ± 0.4 | 1.4 ± 0.2 | |
| HlgB Gly130Asp | 5.8 ± 0.7 | 0.5 ± 0.1 | 7.9 ± 0.9 | 2.4 ± 0.3 | |
a
IC50 and Ki(app) are defined in Materials and Methods.