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. 2025 Nov 29;16:545. doi: 10.1038/s41598-025-30119-w

Table 3.

ADMET properties of the selected bioactive compounds.

ADMET Esculetin Aloe emodin Chrysophanic acid Aloesin
Absorption
Water solubility −2.497 −3.104 −3.077 −2.309
CaCO2 permeability 0.301 −0.233 1.298 0.305
Intestinal absorption (human) 86.291 74.179 96.558 46.247
Skin permeability −2.796 −2.743 −2.83 −2.736
P-glycoprotein substrate Yes Yes Yes Yes
P-glycoprotein I inhibitor No No No No
P-glycoprotein II inhibitor No No No No
Distribution
VDss (human) 0.528 0.671 0.272 0.212
Fraction unbound (human) 0.484 0.226 0.154 0.416
BBB permeability 0.025 −0.729 0.212 −1.29
CNS permeability −2.296 −2.466 −2.111 −3.783
Metabolism
CYP2D6 substrate No No No No
CYP3A4 substrate No No No No
CYP1A2 inhibitor Yes Yes Yes No
CYP2C19 inhibitor No No No No
CYP2C9 inhibitor No No No No
CYP2D6 inhibitor No No No No
CYP3A4 inhibitor No No No No
Excretion
Total clearance 0.671 0.008 0.02 0.456
Renal OCT2 substrate No No No No
Toxicity
AMES toxicity No Yes Yes No
Max. tolerated dose (human) −0.262 −0.089 −0.256 0.437
hERG I inhibitor No No No No
hERG II inhibitor No No No No
Oral rat acute toxicity (LD50) 2.337 2.329 2.275 2.47
Oral rat chronic toxicity (LOAEL) 1.504 1.878 2.057 3.95
Hepatotoxicity No No No Yes
Skin sensitisation No No No No
T.Pyriformis toxicity 0.39 0.563 0.794 0.285
Minnow toxicity 2.341 2.337 1.603 4.394